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Author |
Thread Statistics | Show CCP posts - 17 post(s) |
Galaxxis
Caldari Provisions Caldari State
4
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Posted - 2016.03.12 22:00:29 -
[61] - Quote
Nick Kanjus wrote:I'm a bit confused about this one. I might be totally wrong but when I read the description of cytoplasm it says: Seen throughout all the whole cell, except in the nucleus (blue marker). The intensity can vary throughout the cell, and is often stronger close to the nucleus. in other words: all the red might be green but the blue is blue without a spec of green in it. Now I got image 100096917 (as in the screenshot here: http://i.imgur.com/cVv5kwc.png ). Basically its green all over so I figured the sample was useless. But to my surprise 50% match was on cytoplasm. Am I misunderstanding cytoplasm and reject my samples to soon. Or did 50% of the people not read the first line of the description?
I've seen ones with most people voting cytoplasm even if there's actually no green at all in the red part. It seems like everyone just picks that on every slide because they think everyone else is going to pick it as well. |
Gilbaron
Free-Space-Ranger Northern Coalition.
1901
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Posted - 2016.03.12 22:10:52 -
[62] - Quote
it's hard to say without the color filters
keep in mind that it can be cytoplasm and nucleoplasm/nucleoplasm in the same picture.
it leans towards cytoplasm, if the red area is well stained with the green marker, maybe, but not necessarily concentraded around the blue area, but can also be equally spread throughout the whole cell. add vesicles if there are bright green dots.
it leans towards nucleoplasm/nucleus if only the blue part is stained.
in any case, you need a visible difference between the two areas to mark either. if there is NO difference, pick weak or undefined.
if there is only very weak staining in the red area, don't pick cytoplasm.
i would say that's plasma membrane in your picture since the green marker spills outside of the red one. it could be more than that, but that's not really visible without the filters.
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Nick Kanjus
Lone Star Warriors Yulai Federation
9
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Posted - 2016.03.12 22:17:12 -
[63] - Quote
Gilbaron wrote:it's hard to say without the color filters
keep in mind that it can be cytoplasm and nucleoplasm/nucleoplasm in the same picture.
I'm a bit confused about that. Nucleoplasm/nucleoplasm demands that there is some sort of green in the blue area. While cytoplasm forbids it . |
Gilbaron
Free-Space-Ranger Northern Coalition.
1901
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Posted - 2016.03.12 22:30:36 -
[64] - Quote
you can have both at the same time. you just need a visible difference between the two areas to make a distinction.
i'm trying to find an example for you right now, give me a few minutes :) |
Gilbaron
Free-Space-Ranger Northern Coalition.
1901
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Posted - 2016.03.12 23:09:08 -
[65] - Quote
took me a while to find a good one, but here we go:
green: https://gyazo.com/fccb98747601e1572971c0e1fd821ffd
blue: https://gyazo.com/0b43756d9a4dd0bfa55ea1ea4af46f99
red: https://gyazo.com/9b0b960f63f7de49f49752ce3308b6bd
green+blue: https://gyazo.com/2f505174801640b6add614f8c13784c7
the green marker is very well visible throughout the entire cell, however, it's much stronger in the nucleus (blue marker).
the holes in the nucleus aren't very pronounced, but if you look closely, you can see them. they match up with the green marker, therefore we are checking the "nucleoplasm" option
the consensus on this one is 100% nucleoplasm, 90% cytoplasm and 10% plasmatic membrane.
i don't think the plasmatic membrane option is correct, since the green staining can not be seen clearly outside the boundaries set by the red marker
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Nick Kanjus
Lone Star Warriors Yulai Federation
9
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Posted - 2016.03.12 23:23:48 -
[66] - Quote
Gilbaron wrote:took me a while to find a good one, but here we go:
the consensus on this one is 100% nucleoplasm, 90% cytoplasm and 10% plasmatic membrane.
i don't think the plasmatic membrane option is correct, since the green staining can not be seen clearly outside the boundaries set by the red marker
Hey man first off thank you for taking the effort to explain this ;)
I would fully agree to you about the Nucleoplasm, your description fits the way I look at it. I also agree that the plasmatic membrane is incorrect. But I disagree to the 90% cytoplasm result.
To quote myself, cytoplasm: 'all the red might be green but the blue is blue without a spec of green in it.' Your imagine is showing green all over the cells (including the blue area's). shouldn't that mean it is defiantly NOT cytopalsm |
Galaxxis
Caldari Provisions Caldari State
4
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Posted - 2016.03.13 00:23:37 -
[67] - Quote
http://i.imgur.com/131ujOT.png
Here's one with a ton of stuff going on. It has a cytokinetic bridge, an organizing center and spaghetti!! |
Gilbaron
Free-Space-Ranger Northern Coalition.
1901
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Posted - 2016.03.13 00:36:13 -
[68] - Quote
@Nick
once again. you can have multiple things going on at the same time. the green staining in the blue area comes from nucleoplasm, whereas the green staining in the red area comes from cytoplasm
this is an example of pure cytoplasm (+ vesicles)
https://gyazo.com/597b71ec73291b5b1f0fce9b64ec7cd4
the faint green spots in this zoom from the middle section
https://gyazo.com/d0bf894db0a7f870772ca1caf7917045
is just from more cytoplasm that is on top or below the nucleus. (you are looking at a 2D image of a 3D structure) |
Beta Maoye
105
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Posted - 2016.03.13 02:26:21 -
[69] - Quote
I have a case that fit both Nucleoplasm and Nucleoli, but these two options are exclusive in the mini game. Green and blue channel: http://uploadpie.com/LiGB6 Green channel: http://uploadpie.com/eEVpE Blue channel: http://uploadpie.com/XqAqA |
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HPA Illuminator
State War Academy Caldari State
25
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Posted - 2016.03.13 07:28:33 -
[70] - Quote
Gilbaron wrote:https://i.gyazo.com/54efcad7e2f4666f6ad66988a2e17ceb.png
Was this a question? I would say it's either cell junctions of plasma membrane. I would lean towards plasma membrane because in the upper left and lower right parts you can see some staining outside the cells. Possibly both CJ+PM is correct, that's also an option :) |
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HPA Illuminator
State War Academy Caldari State
25
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Posted - 2016.03.13 07:32:01 -
[71] - Quote
Nick Kanjus wrote:I'm a bit confused about this one. I might be totally wrong but when I read the description of cytoplasm it says: Seen throughout all the whole cell, except in the nucleus (blue marker). The intensity can vary throughout the cell, and is often stronger close to the nucleus. in other words: all the red might be green but the blue is blue without a spec of green in it. Now I got image 100096917 (as in the screenshot here: http://i.imgur.com/cVv5kwc.png ). Basically its green all over so I figured the sample was useless. But to my surprise 50% match was on cytoplasm. Am I misunderstanding cytoplasm and reject my samples to soon. Or did 50% of the people not read the first line of the description? EDIT: same on 100021233: http://i.imgur.com/wRnL8Sq.png that one is even fully conflicting with both Nucleus and Cytoplasm
Multiple choices are ok. And you should really try toggling all colors on/off and look at green only, green+red, green+blue.
This is probably helpful: https://www.youtube.com/watch?v=PW5Yl6MjZjk&feature=youtu.be |
HPA Illuminator
State War Academy Caldari State
25
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Posted - 2016.03.13 07:37:03 -
[72] - Quote
There are some cases like that, and if the nucleoli is prominent, i'd go for nucleus + nucleoli.
In this case, I don't think the overlap is good, it looks like nucleoplasm+speckles to me (just like you've chosen). |
Galaxxis
Caldari Provisions Caldari State
5
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Posted - 2016.03.13 15:36:46 -
[73] - Quote
http://i.imgur.com/bAPqPdI.jpg
Oh no!!! Something ate the aggresome! |
HPA Illuminator
State War Academy Caldari State
25
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Posted - 2016.03.13 16:04:32 -
[74] - Quote
lol pacman hungry |
Helios Anduath
Signal Cartel EvE-Scout Enclave
117
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Posted - 2016.03.13 16:07:07 -
[75] - Quote
mmmmmm, chocolate coated aggresome |
Hal Morsh
Hmmzor. Muffins of Mayhem
514
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Posted - 2016.03.13 16:43:34 -
[76] - Quote
But I bet it tells you two of the options are wrong, and 60% choose cytoplasm, because if project discovery isn't outright glitching it's most people selecting cytoplasm.
Problems, Problems.
Q: How many EVE players does it take to change a lightbulb?
A: CHANGE???????? NNOOOOOOOOOOO
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Selphentine
Pastafaris
1
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Posted - 2016.03.13 16:45:10 -
[77] - Quote
Nick Kanjus wrote:Gilbaron wrote:took me a while to find a good one, but here we go:
the consensus on this one is 100% nucleoplasm, 90% cytoplasm and 10% plasmatic membrane.
i don't think the plasmatic membrane option is correct, since the green staining can not be seen clearly outside the boundaries set by the red marker
Hey man first off thank you for taking the effort to explain this ;) I would fully agree to you about the Nucleoplasm, your description fits the way I look at it. I also agree that the plasmatic membrane is incorrect. But I disagree to the 90% cytoplasm result. To quote myself, cytoplasm: 'all the red might be green but the blue is blue without a spec of green in it.' Your imagine is showing green all over the cells (including the blue area's). shouldn't that mean it is defiantly NOT cytopalsm
As far as i know, this thing is also not purely 2 dimensional. you may have cytoplasma under/over a core, giving it a green look and way less green, but still green, in the core. (in general, not for this example.) |
Nick Kanjus
Lone Star Warriors Yulai Federation
9
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Posted - 2016.03.13 17:50:38 -
[78] - Quote
Selphentine wrote:took me a while to find a good one, but here we go:
As far as i know, this thing is also not purely 2 dimensional. you may have cytoplasma under/over a core, giving it a green look and way less green, but still green, in the core. (in general, not for this example.)
with that mindset things would start making more sense. If I'm allowed to hide the green with the blue then it could indeed be both at the same time. I still find it somewhat strange but seeing as most people do it and that most results of the test server where correct....I must have been wrong.
Thanks to you (and the others) for all the help :)
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Beta Maoye
108
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Posted - 2016.03.14 18:08:51 -
[79] - Quote
I think this slide has two signals, microtubule organizing center and centrosome, not just microtubule organizing center. RBG: http://uploadpie.com/onin4 Zoom 1: http://uploadpie.com/IHDCU Zoom 2: http://uploadpie.com/wRlX5 Zoom 3: http://uploadpie.com/VlkIU |
Helios Anduath
Signal Cartel EvE-Scout Enclave
122
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Posted - 2016.03.14 19:09:56 -
[80] - Quote
They don't really look like Centrosome because they are not two very well defined spots side by side. I would just have gone MTOC for this. |
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March rabbit
Federal Defense Union
1707
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Posted - 2016.03.14 19:21:36 -
[81] - Quote
I've heard that some images was analyzed by professionals? screenshot
The Mittani: "the inappropriate drunked joke"
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HPA Illuminator
State War Academy Caldari State
26
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Posted - 2016.03.14 22:05:02 -
[82] - Quote
Helios Anduath wrote:They don't really look like Centrosome because they are not two very well defined spots side by side. I would just have gone MTOC for this.
Based on zoom 2 I'd go for centrsosome and think the others cells were slightly out of focus. |
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HPA Illuminator
State War Academy Caldari State
26
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Posted - 2016.03.14 22:05:40 -
[83] - Quote
March rabbit wrote:I've heard that some images was analyzed by professionals? screenshot
Thanks for noticing, will double check it and correct if necessary. |
beakerax
Pator Tech School
230
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Posted - 2016.03.15 01:54:38 -
[84] - Quote
ummm http://i.imgur.com/3AVfx0H.jpg (#100078311) |
Galaxxis
Caldari Provisions Caldari State
6
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Posted - 2016.03.15 02:48:45 -
[85] - Quote
I had that one yesterday! I put cytoskeleton, although I'm really not sure if that's right. |
Memphis Baas
1338
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Posted - 2016.03.15 03:06:14 -
[86] - Quote
I had that one just now; the splash of green overlaps the nucleus on every cell, so it can't really be anything outside the nucleus. I put Nuclear Membrane, Abnormal sample.
A Google images for "abnormal nuclear membrane stain" shows a couple examples where the membrane could fold in on itself or maybe shrink, to look slightly like that. |
Galaxxis
Caldari Provisions Caldari State
6
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Posted - 2016.03.15 04:16:38 -
[87] - Quote
Memphis Baas wrote:I had that one just now; the splash of green overlaps the nucleus on every cell, so it can't really be anything outside the nucleus. I put Nuclear Membrane, Abnormal sample. A Google images for "abnormal nuclear membrane stain" shows a couple examples where the membrane could fold in on itself or maybe shrink, to look slightly like that.
I mean I agree that it kind of looks like that, and that was my thought as well, but then why is there still a nice round blue part visible? Shouldn't that be all scrunched up inside the green part? |
Galaxxis
Caldari Provisions Caldari State
6
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Posted - 2016.03.15 07:03:48 -
[88] - Quote
http://i.imgur.com/DGmPo87.png
I get these sometimes, but usually there's no dye anywhere except for a single bundle of microtubule ends somewhere. This one has green goo all over the place. |
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HPA Illuminator
State War Academy Caldari State
26
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Posted - 2016.03.15 07:07:20 -
[89] - Quote
Memphis Baas wrote:I had that one just now; the splash of green overlaps the nucleus on every cell, so it can't really be anything outside the nucleus. I put Nuclear Membrane, Abnormal sample. A Google images for "abnormal nuclear membrane stain" shows a couple examples where the membrane could fold in on itself or maybe shrink, to look slightly like that.
<3
Something happened to cells during prepp, so what you see is not something that is actually specific. I don't think it't the nuclear membrane but rather the plasma membrane that has shrunk (the nucleus still intact).
Have added to checkup list. |
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HPA Illuminator
State War Academy Caldari State
26
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Posted - 2016.03.15 07:08:39 -
[90] - Quote
Galaxxis wrote:http://i.imgur.com/DGmPo87.pngI get these sometimes, but usually there's no dye anywhere except for a single bundle of microtubule ends somewhere. This one has green goo all over the place.
I'd say the green mostly is specific, and I'll be curious to see the results of it (I think some cells have been washed away during sample prep). |
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